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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Spatial analysis > Takara Trekker U Single-cell Spatial Mapping Kit
True single cell °ø°£ ºÐ¼®ÀÌ °¡´ÉÇÑ reagent kit

Takara Trekker U Single-cell Spatial Mapping Kit

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Trekker U 10x10 Bundle
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Trekker Starter Kit Bundle (UV lamp)
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Trekker 10x10 Training Kit Bundle
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  • ½ÇÁ¦ single cellÀÇ ÇÙÀ» ÀÌ¿ëÇÑ °ø°£(spatial) transcriptome ºÐ¼® ½Ã¾à
  • Single cell ºÐ¼® µ¥ÀÌÅÍ¿¡ À§Ä¡ Á¤º¸¸¦ ºÎ¿©ÇÏ¿© ÇØ¼®
  • ¼¼Æ÷³» ÇÙÀ» DNA barcode·Î taggingÇÏ´Â ¹æ½ÄÀ¸·Î º°µµÀÇ segmentation ¾øÀÌ Á¤È®ÇÑ °ø°£Çؼ® °¡´É
  • º» Á¦Ç°¿¡¼­ ÃßÃâÇÑ ÇÙÀ» ÀÌ¿ëÇÏ¿© ±âÁ¸ÀÇ single cell ºÐ¼® ½Ã½ºÅÛÀÇ ¿öÅ©Ç÷ο쿡 Àû¿ë °¡´É
  • º°µµ·Î °í°¡ÀÇ °ø°£ ÇØ¼® ÀåÄ¡³ª probe set ºÒÇÊ¿ä: Á¾¿¡ »ó°ü¾øÀÌ ºÐ¼® °¡´É
  • Broad Institute¿¡¼­ °³¹ßµÈ Slide tags[1] ±â¼úÀ» »ó¿ëÈ­ÇÑ Á¦Ç°
    [1] Nature Vol 625, pp. 101-109 (2024).
  • Trekker analysis pipeline Á¦°ø : Linux ½Ã½ºÅÛ ÀÌ¿ë
    - User manual ´Ù¿î·Îµå
´Ù¾çÇÑ Application protocol
1. Trekker U Library Preparation with 10x Multiome Kit Protocol-At-A-Glance
2. Trekker U Library Preparation with BD Rhapsody Single-Cell ATAC-Seq and mRNA WTA Kit Protocol-At-A-Glance
3. Trekker U Library Preparation with Illumina Single Cell 3' RNA Prep Kits Protocol-At-A-Glance
4. Trekker and Seeker Preserving and Sectioning Tissue Protocol-At-A-Glance
5. Trekker Cardiac Tissue Dissociation Protocol-At-A-Glance
6. Trekker Library Preparation with 10x Chromium 3' Reagent Kit Protocol-At-A-Glance
7. Trekker Library Preparation with the BD Rhapsody WTA Kit Protocol-At-A-Glance
8. Trekker Neuronal Tissue Dissociation Protocol-At-A-Glance
Á¦Ç°¼³¸í
Trekker´Â single cell µ¥ÀÌÅÍ¿¡ À§Ä¡(spatial) Á¤º¸¸¦ ºÎ¿©ÇÏ¿© ÇØ¼®ÀÌ °¡´ÉÇϵµ·Ï °³¹ßÇÑ ½ÇÁ¦ single cell spatial transcriptome ºÐ¼® ½Ã¾àÀÌ´Ù.
º» Á¦Ç°Àº tileÀ̶ó ºÎ¸£´Â ½½¶óÀ̵å À§¿¡ DNA barcode°¡ °áÇÕµÈ bead(Á÷°æ 10 §­)¸¦ ºÙ¿© »ç¿ëÇÑ´Ù. ÀÌ tile¿¡ µ¿°á ÀýÆí (fresh frozen tissue)À» ¿Ã·Á ¼¼Æ÷¿Í bead°¡ Á¢ÃËÇϵµ·Ï Çϰí, UV¸¦ Á¶»çÇÏ¿© bead·ÎºÎÅÍ ºÐ¸®µÈ DNA barcode°¡ ¼¼Æ÷ÀÇ ÇÙ¿¡ taggingÇÏ´Â ¿ø¸®¸¦ ÀÌ¿ëÇÑ´Ù. ÀÌÈÄ ÇÙÀ» ºÐ¸®ÇÏ¿© RNA sequencing(snRNA-Seq)À» ½Ç½ÃÇÏ¿©, °¢°¢ÀÇ ÇÙ¿¡¼­ À¯·¡ÇÑ À¯ÀüÀÚ Á¤º¸ ¹× À§Ä¡ Á¤º¸¸¦ Æ÷ÇÔÇÑ Àü¹ÝÀûÀÎ ÇØ¼®ÀÌ °¡´ÉÇÏ´Ù.


[±×¸²] Trekker Workflow
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Code

Á¦Ç°¸í

SK017

Trekker U 10x10 Bundle (4 ȸ)

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Trekker Reagent Box 1

Trekker 10x10 Reaction Pouch

Trekker 10x10 Tile Masking Sticker

Trekker 10x10 Wash Chambers

Trekker U 10x10 Tiles

Trekker O Ring

K011

Trekker Starter kit bundle (UV lamp)*

±¸¼ºÇ°

Trekker UV lamp holder

UV lamp

UV lamp driver

UV lamp power supply

SK020

Trekker 10x10 Training Kit Bundle**

±¸¼ºÇ°

Trekker Reagent Box 1

Trekker 10x10 Tile Masking Sticker

Trekker O Ring

Trekker 10x10 Wash Chambers

10x10 Training Tiles


* Trekker Starter Kit Bundle (UV lamp)Àº Trekker Àü¿ë UV lamp¸¦ Æ÷ÇÔÇÑ Á¦Ç°À¸·Î, ÃÖÃÊ ¼¼ÆÃ½Ã 1ȸ¸¸ ±¸¸Å
** Trekker 10x10 Training Kit Bundle ¿¡´Â ÃÖÃÊ ¼¼ÆÃ ȤÀº »õ·Î¿î Á¶Á÷¿¡ Àû¿ëÇϰíÀÚ ÇÒ ¶§ »ç¿ëÇÒ ¼ö ÀÖ´Â training tileÀÌ Æ÷ÇԵǾî ÀÖÀ½.
Àû¿ë°¡´ÉÇÑ application ¹× protocol
Applications and workflows
 
Application Compatible single-cell or multiomic workflow Compatible single nucleus capture and library preparation protocol Compatible Trekker Tile

Spatial 3' gene expression

10x Chromium 3¡Ç RNA, v3.1, v4

Trekker Library Preparation with 10x Chromium 3¡Ç Reagent Kit Protocol-At-A-Glance

Trekker U

BD Rhapsody WTA

Trekker Library Preparation with BD Rhapsody WTA Kit Protocol-At-A-Glance

Trekker U

Illumina Single Cell 3¡¯ RNA Prep

Trekker U Library Preparation with Illumina Single Cell 3¡¯ RNA Prep Kits Protocol-At-A-Glance

Trekker U

Parse Evercode WT v3

Trekker Q Library Preparation with Parse Evercode WT v3 Kits Protocol-At-A-Glance

Trekker Q
[coming soon]

Spatial immune profiling

BD Rhapsody Single-Cell TCR/BCR Next and mRNA Whole Transcriptome Analysis

Trekker Library Preparation with BD Rhapsody scTCR/BCR Next and mRNA Whole Transcriptome Analysis User Guide (User demonstrated)

Trekker U

Spatial epigenomics

BD Rhapsody Single-Cell ATAC-Seq and mRNA Whole Transcriptome Analysis

Trekker U Library Preparation with BD Rhapsody Single-Cell ATAC-Seq and mRNA WTA Kit Protocol-At-A-Glance

Trekker U

10x Genomics Epi Multiome ATAC + Gene Expression

Trekker U Library Preparation with 10x Multiome Kit Protocol-At-A-Glance Trekker U

Tissue preparation protocols
Preparation Tissue type Compatible tissue preparation and dissociation protocol
Standard protocol For most non-fibrous mammalian tissues; recommended starting point for most optimizations Trekker and Seeker Preserving and Sectioning Tissue Protocol-At-A-Glance
Neuronal protocol For myelin protein removal from neuronal tissues where myelin fibers are seen in debris outside nucleus Trekker Neuronal Tissue Dissociation Protocol-At-A-Glance
Cardiac, tumor, and other fibrous tissue protocol For many fibrous tissues, including cardiac and skeletal muscle, as well as many tumor types Trekker Cardiac Tissue Dissociation Protocol-At-A-Glance

Keyword :

Spatial analysis
>
Àü¿ë ÀåÄ¡ ÇÊ¿ä ¾øÀÌ whole transcriptome °ø°£..
Takara Seeker Spatial Transcriptomics Kit
>
True single cell °ø°£ ºÐ¼®ÀÌ °¡´ÉÇÑ reagent ..
Takara Trekker U Single-cell Spatial Mapping Kit