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Gene transfer (virus)
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Retrovirus
> Integration Site Analysis Kit
Integration Site Analysis Kit
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Clontech
631467
Retro-X Integration Site Analysis Kit
each
(Package)
1,458,400¿ø
1,823,000¿ø
11.01 ~ 12.27
,
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
RetroNectin¢ç Pro (Rec..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
GenomeWalker¸¦ ±â¹ÝÀ¸·Î provirusÀÇ °Ô³ð »ðÀÔ ºÎÀ§¸¦ ½Äº°
MMLV ¹× MSCVŸÀÔÀÇ retroviral vector¿¡ Àû¿ë °¡´É
ºÐ¼®¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾à Æ÷ÇÔ
Á¦Ç°¼³¸í
Retro-X Integration Site Analysis Kit´Â ·¹Æ®·Î ¹ÙÀÌ·¯½º º¤ÅÍ°¡ host genome DNA¿¡ provirus·Î »ðÀÔµÈ ºÎºÐÀ» GenomeWalker ±â¼úÀ» ±âºÐÀ¸·Î ºÐ¼®Çϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù. ·¹Æ®·Î¹ÙÀÌ·¯½º´Â RNA genome virus·Î DNA·Î ¿ªÀü»ç µÇ¾î host genome¿¡ »ðÀԵȴÙ. ¹ÙÀÌ·¯½º DNA´Â host genome¿¡ ¹«ÀÛÀ§·Î »ðÀԵDZ⠶§¹®¿¡ ¸ñÀûÀ¯ÀüÀÚ ¹× endogenous À¯ÀüÀÚ ¹ßÇö¿¡ ¿µÇâÀ» ÁÙ ¼ö ÀÖ´Ù. µû¶ó¼ provirusÀÇ »ðÀÔºÎÀ§ÀÇ ½Äº°Àº host cellÀÇ Ç¥ÇöÇü ¿¬±¸¿¡ Áß¿äÇÏ´Ù. ÀÌ Å°Æ®¿¡´Â ½ÇÇè¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ´Ù. (ÀÚ¼¼ÇÑ ±¸¼ºÇ° ¸®½ºÆ®´Â CoA¸¦ Âü°í)
GenomeWalker °³¿ä
1. ¹ÙÀÌ·¯½º¸¦ ÇüÁú µµÀÔÇÑ ¼¼Æ÷¿¡¼ ÃßÃâÇÑ genomic DNA¸¦ 3Á¾·ùÀÇ blunt-end Á¦ÇÑÈ¿¼Ò ó¸®
2. ÆòÈ°¸»´ÜÀÌ µÈ genomic DNA library¿¡ GenomeWalker adaptor¸¦ ligation.
3. Library¿¡¼ adaptor¿Í ·»Æ¼¹ÙÀÌ·¯½º ½ÃÄý½º°¡ Æ÷ÇÔµÈ ºÎºÐÀ» PCR ÁõÆø
4. Nested PCRÀ» ÅëÇØ ´ÜÀϹêµå ÁõÆø, ÁõÆø µÈ »ê¹°Àº sequencingÀ» ÅëÇؼ integrationºÎºÐ ºÐ¼®, º¹¼öÀÇ ´ÜÆíÀÌ ÁõÆøµÇ´Â °æ¿ì¿¡´Â
°¢°¢ÀÇ band¸¦ Á¤Á¦ ÇÑ ÈÄ sequencingÀ» ÅëÇؼ ºÐ¼®
±×¸². Flow chart of the Lenti-X Integration Site Analysis protocol. The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result. Lane 1: HpaI library. Lane 2: DraI library. Lane 3: SspI library. Lane 4: No template control. Lane M: DNA size markers. AP: Adaptor primer. LSP: Lentivirus-specific primer.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit