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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > Lentivirus ³óÃà ½Ã¾à
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£´ÜÈ÷ ³óÃà

Lentivirus ³óÃà ½Ã¾à

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631231
Lenti-X¢â Concentrator
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
494,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32764
Clontech
631232
Lenti-X¢â Concentrator
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ml
1,706,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • ÃÊ°í¼Ó¿ø½ÉºÐ¸® ¾øÀÌ ¹ÙÀÌ·¯½º ¿ª°¡¸¦ 100 ¹è±îÁö Áõ°¡½ÃÅ´
  • °£ÆíÇÑ Á¶ÀÛ°úÁ¤ - mix and spin
  • ¹ø°Å·Î¿î °úÁ¤ ¾øÀÌ ½±°Ô ´ë¿ë·® Àû¿ë °¡´É
  • ÃÊ°í¼Ó¿ø½ÉºÐ¸® ºÒÇÊ¿ä
Lentivirus ³óÃàÀ» À§ÇÏ¿© ¹ø°Å·Î¿î ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â¸¦ »ç¿ëÇÏ°í ½ÍÁö ¾ÊÀ» °æ¿ì Lenti-XTM Concentrator¸¦ »ç¿ëÇÏ¿© 100 ¹è±îÁö ¿ª°¡¸¦ Áõ°¡½Ãų ¼ö ÀÖÀ¸¸ç ³ôÀº MOI·Î ¸ñÀû ¼¼Æ÷¿¡ °¨¿°½Ãų ¼ö ÀÖ´Ù.

°£´ÜÇÑ Á¶ÀÛ°úÁ¤ (Mix, Wait, Spin)
Lenti-X Concentrator´Â ºü¸£°í °£´ÜÇϸç È¿À²ÀûÀÎ lentivirus ³óÃà ¹æ¹ýÀÌ´Ù. ½ÇÇè°úÁ¤Àº lentivirus »óÃþ¾×¿¡ Lenti-X Concentrator ½Ã¾àÀ» ¼¯¾î Àá±ñ µ¿¾ÈÀÇ ¹ÝÀÀ ÈÄ ÀÏ¹Ý ¿ø½ÉºÐ¸®±â·Î spin down ÇÏ´Â 3 ´Ü°è·Î ¸Å ¿ì °£´ÜÇÏ´Ù(Figure 1). 1 ½Ã°£ À̳»¿¡ 100 ¹è±îÁö ¿ª°¡¸¦ Áõ°¡½Ãų ¼ö ÀÖ À¸¸ç, ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â¸¦ »ç¿ëÇÏÁö ¾Ê°íµµ 1 ½Ã°£ À̳»¿¡ 100 ¹è±îÁö ¿ª°¡¸¦ Áõ°¡½Ãų¼ö ÀÖ´Ù. Lenti-X concentrator´Â ClontechÀÇ Lenti-X Expression & Packaging SystemÀ¸·Î ¸¸µé¾îÁø virus »Ó¸¸ ¾Æ´Ï¶ó ´Ù¸¥ ¸ðµç lentivirus »óÃþ¾×À» ³óÃàÇÒ ¼ö ÀÖÀ¸¸ç ¿øÇÏ´Â ºÎÇÇ¿Í ³óµµ·Î ½±°Ô Á¶ÀýÇÒ ¼ö ÀÖ´Ù.
Lenti-X¢â Concentrator »ç¿ë ¹æ¹ý
100 ¹è±îÁö ¿ª°¡ »ó½Â
Lenti-X Concentrator Á¶ÀÛ °úÁ¤À» »ç¿ëÇϸé lentivirus¸¦ º»·¡ ºÎÇÇÀÇ 1/100 ·Î ÁÙÀÏ ¼ö ÀÖ°í, 90%ÀÇ È¸¼öÀ²·Î 107 ~ 109 IFU/ml·Î ¿ª°¡¸¦ »ó½Â½Ãų ¼ö ÀÖ´Ù (Figure 2).
¾î¶² ºÎÇÇÀÇ »óÃþ¾×À¸·Îµµ À¯»çÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
ÃÊ°í¼Ó¿ø½ÉºÐ¸®º¸´Ù ÈξÀ °£´ÜÇÑ ¹æ¹ý
Lenti-X Concentrator¿Í ÃÊ°í¼Ó¿ø½ÉºÐ¸®¸¦ ÀÌ¿ëÇÑ ¹ÙÀÌ·¯½º ³óÃà ¹æ¹ýÀ» ºñ±³Çϸé Lenti-X ¹ýÀÇ ÀåÁ¡ÀÌ È®¿¬È÷ µé¾î³­´Ù (Table I). Lenti-X Concentrator ´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸®¹ýº¸´Ù ´õ¿í À¯¿¬ÇÏ°í ºü¸£¸ç, ½¬¿ï »Ó¸¸ ¾Æ´Ï¶ó ¸Å¿ì È¿°úÀûÀÌ´Ù. ¹ÙÀÌ·¯½º »óÃþ¾×ÀÇ ºÎÇǸ¦ ÁÙÀ̰ųª ¿ª°¡¸¦ Áõ°¡½ÃÄÑ¾ß ÇÒ ¶§, Lenti-X Concentrator´Â ¹ø°Å·Ó°í ½Ã°£ÀÌ ¸¹ÀÌ ¼Ò¿äµÇ´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸® °úÁ¤ ¾øÀÌ ºü¸£°í °£´ÜÇÏ°Ô ³óÃà½Ãų ¼ö ÀÖ´Ù.


Figure 1. The Lenti-X Concentrator protocol. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4¡ÆC, and spin. That¡¯s it.
Table I: Lenti-X Concentrator vs. Ultracentrifugation
Feature Lenti-X Concentrator Ultracentrifugation
Easily Scalable Yes No
Specialized Equipment No Yes
Time Required ~1 hr 4 hr to overnight
Ease-of-Use ++++ +
Yield £¾90% £¾90%


Figure 2. Efficient concentration with minimal loss. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30¥ìl using the Lenti-X Concentrator Reagent and reflects a 100-fold increase in viral titer. The total amount of virus contained in each sample is also indicated, which shows that the resuspended pellet captured 90% of the virus present in original sample. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.




Keyword : concentrator,soeasy,so easy

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