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Gene transfer (virus)
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Lentivirus
> Lentivirus ³óÃà ½Ã¾à
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£´ÜÈ÷ ³óÃà
Lentivirus ³óÃà ½Ã¾à
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(ºÎ°¡¼¼º°µµ)
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Clontech
631231
Lenti-X¢â Concentrator
100 ml
494,000¿ø
Clontech
631232
Lenti-X¢â Concentrator
500 ml
1,706,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
ÃÊ°í¼Ó¿ø½ÉºÐ¸® ¾øÀÌ ¹ÙÀÌ·¯½º ¿ª°¡¸¦ 100 ¹è±îÁö Áõ°¡½ÃÅ´
°£ÆíÇÑ Á¶ÀÛ°úÁ¤ - mix and spin
¹ø°Å·Î¿î °úÁ¤ ¾øÀÌ ½±°Ô ´ë¿ë·® Àû¿ë °¡´É
ÃÊ°í¼Ó¿ø½ÉºÐ¸® ºÒÇÊ¿ä
Lentivirus ³óÃàÀ» À§ÇÏ¿© ¹ø°Å·Î¿î ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â¸¦ »ç¿ëÇÏ°í ½ÍÁö ¾ÊÀ» °æ¿ì Lenti-XTM Concentrator¸¦ »ç¿ëÇÏ¿© 100 ¹è±îÁö ¿ª°¡¸¦ Áõ°¡½Ãų ¼ö ÀÖÀ¸¸ç ³ôÀº MOI·Î ¸ñÀû ¼¼Æ÷¿¡ °¨¿°½Ãų ¼ö ÀÖ´Ù.
°£´ÜÇÑ Á¶ÀÛ°úÁ¤ (Mix, Wait, Spin)
Lenti-X Concentrator´Â ºü¸£°í °£´ÜÇϸç È¿À²ÀûÀÎ lentivirus ³óÃà ¹æ¹ýÀÌ´Ù. ½ÇÇè°úÁ¤Àº lentivirus »óÃþ¾×¿¡ Lenti-X Concentrator ½Ã¾àÀ» ¼¯¾î Àá±ñ µ¿¾ÈÀÇ ¹ÝÀÀ ÈÄ ÀÏ¹Ý ¿ø½ÉºÐ¸®±â·Î spin down ÇÏ´Â 3 ´Ü°è·Î ¸Å ¿ì °£´ÜÇÏ´Ù(Figure 1). 1 ½Ã°£ À̳»¿¡ 100 ¹è±îÁö ¿ª°¡¸¦ Áõ°¡½Ãų ¼ö ÀÖ À¸¸ç, ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â¸¦ »ç¿ëÇÏÁö ¾Ê°íµµ 1 ½Ã°£ À̳»¿¡ 100 ¹è±îÁö ¿ª°¡¸¦ Áõ°¡½Ãų¼ö ÀÖ´Ù. Lenti-X concentrator´Â ClontechÀÇ Lenti-X Expression & Packaging SystemÀ¸·Î ¸¸µé¾îÁø virus »Ó¸¸ ¾Æ´Ï¶ó ´Ù¸¥ ¸ðµç lentivirus »óÃþ¾×À» ³óÃàÇÒ ¼ö ÀÖÀ¸¸ç ¿øÇÏ´Â ºÎÇÇ¿Í ³óµµ·Î ½±°Ô Á¶ÀýÇÒ ¼ö ÀÖ´Ù.
Lenti-X¢â Concentrator »ç¿ë ¹æ¹ý
100 ¹è±îÁö ¿ª°¡ »ó½Â
Lenti-X Concentrator Á¶ÀÛ °úÁ¤À» »ç¿ëÇϸé lentivirus¸¦ º»·¡ ºÎÇÇÀÇ 1/100 ·Î ÁÙÀÏ ¼ö ÀÖ°í, 90%ÀÇ È¸¼öÀ²·Î 10
7
~ 10
9
IFU/ml·Î ¿ª°¡¸¦ »ó½Â½Ãų ¼ö ÀÖ´Ù (Figure 2).
¾î¶² ºÎÇÇÀÇ »óÃþ¾×À¸·Îµµ À¯»çÇÑ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù.
ÃÊ°í¼Ó¿ø½ÉºÐ¸®º¸´Ù ÈξÀ °£´ÜÇÑ ¹æ¹ý
Lenti-X Concentrator¿Í ÃÊ°í¼Ó¿ø½ÉºÐ¸®¸¦ ÀÌ¿ëÇÑ ¹ÙÀÌ·¯½º ³óÃà ¹æ¹ýÀ» ºñ±³Çϸé Lenti-X ¹ýÀÇ ÀåÁ¡ÀÌ È®¿¬È÷ µé¾î³´Ù (Table I). Lenti-X Concentrator ´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸®¹ýº¸´Ù ´õ¿í À¯¿¬ÇÏ°í ºü¸£¸ç, ½¬¿ï »Ó¸¸ ¾Æ´Ï¶ó ¸Å¿ì È¿°úÀûÀÌ´Ù. ¹ÙÀÌ·¯½º »óÃþ¾×ÀÇ ºÎÇǸ¦ ÁÙÀ̰ųª ¿ª°¡¸¦ Áõ°¡½ÃÄÑ¾ß ÇÒ ¶§, Lenti-X Concentrator´Â ¹ø°Å·Ó°í ½Ã°£ÀÌ ¸¹ÀÌ ¼Ò¿äµÇ´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸® °úÁ¤ ¾øÀÌ ºü¸£°í °£´ÜÇÏ°Ô ³óÃà½Ãų ¼ö ÀÖ´Ù.
Figure 1. The Lenti-X Concentrator protocol
. Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min to overnight at 4¡ÆC, and spin. That¡¯s it.
Table I: Lenti-X Concentrator vs. Ultracentrifugation
Feature
Lenti-X Concentrator
Ultracentrifugation
Easily Scalable
Yes
No
Specialized Equipment
No
Yes
Time Required
~1 hr
4 hr to overnight
Ease-of-Use
++++
+
Yield
£¾90%
£¾90%
Figure 2. Efficient concentration with minimal loss
. Lentiviral supernatant from a pLVX-ZsGreen1 vector was concentrated from 3 ml down to 30¥ìl using the Lenti-X Concentrator Reagent and reflects a 100-fold increase in viral titer. The total amount of virus contained in each sample is also indicated, which shows that the resuspended pellet captured 90% of the virus present in original sample. Samples were titrated on HT1080 cells and analyzed by flow cytometry 48 hr post-transduction.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
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Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
ÀÓ»ó Ä¡·áÁ¦ °³¹ßÀ» À§ÇÑ
3¼¼´ë Lentivirus packaging system
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter È°¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
½Å°³³ä lentivirus transduction
Lenti-X¢â Transduction Sponge
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System