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PCR
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Standard PCR: Ex, LA, rTaq
> Premix ½Ã¾à (Hot Start Version)
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)
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Takara
R028A
Premix Taq¢â Hot Start Version (500 ¥ìl¡¿5 tube)
100 ȸ
(50 ¥ìl PCR)
96,000¿ø
120,000¿ø
09.02 ~ 10.31
Takara
RR030A
Premix Ex Taq¢â Hot Start Version (500 ¥ìl¡¿5 tube)
100 ȸ
(50 ¥ìl PCR)
120,800¿ø
151,000¿ø
09.02 ~ 10.31
Product index
Overview
PCR FAQs - ÃßõÇÏ´Â PC..
PCR FAQs - Optimizing ..
High Fidelity È¿¼Ò-Pri..
PCR¡¢LA PCR¢â¡¢Hot Sta..
[Cancer Research] HLA ..
½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇÏ..
High fidelity PCR: PrimeST..
Á¤È®ÇÑ GXL DNA Polymer..
GXL Premix ŸÀÔ
ÃÖ°í Á¤È®µµ-Max v2.
ÃÖ°í Á¤È®µµ-Max DNA Po..
񃧯-HS DNA Polymerase
HS (Premix)
HS with GC Buffer
Pyrobest DNA Polymeras..
GXL Fast Premix, dye p..
Standard PCR: Ex, LA, rTaq..
TaKaRa Ex Premier¢â DN..
Ex Taq Hot Start Versi..
TaKaRa Ex Taq
LA Taq Hot Start Versi..
LA Taq
Taq Hot Start Version
Recombinant Taq
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq ..
SpeedSTAR HS È¿¼Ò
Fast PCR¿ë Z-Taq
Taq Antibody
Premix ½Ã¾à (Ex, LA Ta..
Premix ½Ã¾à (Hot Start..
¾÷±×·¹À̵å Taq HS Prem..
Direct PCR: MightyAmp
°ÇÑ ÁõÆø´É·Â- Ver.3
Genotyping¿ë ÃßÃâ½Ã¾à..
񃧯-Ver.2
Dye°¡ Æ÷ÇÔµÈ premix
Colony PCR¿ë premix
Hot Start Version prem..
Fast PCR¿ë premix
PCR¿ë Premix ½Ã¸®Áî
Ư¼ö PCR (Epigenetics, UNG..
EpiTaq HS (for bisulfi..
Multiplex PCR Kit Ver...
¼÷ÁÖÀ¯·¡ DNA ¾ïÁ¦-Taq ..
BcaBEST¢ç DNA Polymera..
TaKaRa Taq¢â HS PCR Ki..
Uracil DNA Glycosylase..
PCR Enzyme (Clontech)
Clontech PCR Enzyme ¼±..
[°³¿ä] So Easy Product
[Cancer Research] Canc..
Advantage 2 Polymerase..
Advantage GC 2 Polymer..
Advantage Genomics LA ..
Advantage HD Polymeras..
Advantage HF 2 PCR Kit
CloneAmp HiFi PCR Prem..
High Fidelity PCR EcoD..
High Yield PCR EcoDry ..
SeqAmp¢â DNA Polymeras..
SuperPlex¢â Premix
Terra PCR Direct Polym..
TITANIUM¢ç Taq DNA Pol..
DNA PCR °ü·Ã Á¦Ç°
Diversify PCR Random M..
LA PCR¢â ¿ë genome DNA..
TaKaRa LA PCR¢â Kit Ve..
TaKaRa PCR Amplificati..
RT-PCR °ü·Ã Á¦Ç°
PrimeScript¢â II High ..
RT-PCR kit, ²Ä²ÄÈ÷ µû..
BcaBEST¢ç RNA PCR Kit ..
PrimeScript¢â High Fid..
PrimeScript¢â One Step..
PrimeScript¢â RT-PCR K..
SMARTer¢ç RACE 5'/3' K..
TaKaRa One Step RNA PC..
TaKaRa RNA LA PCR¢â Ki..
TaKaRa RNA PCR¢â Kit (..
Mutagenesis
Diversify PCR Random M..
PCR Subtraction °ü·Ã Á¦Ç°
Clontech PCR-Select Ba..
Clontech PCR-Select cD..
Clontech PCR-Select Di..
PCR ÀåÄ¡
PCR °ü·Ã ¼Ò¸ðÇ°
TaKaRa PCR Thermal Cyc..
TaKaRa PCR Thermal Cyc..
PCR Accessory Products
Advantage Ultrapure PC..
TaqStart Antibody
Whole Genome SNP Detection
TITANIUM DNA AMPLIFICA..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (PCR)
¸Åµ¶¿ø¼º ´ëÀå±Õ °ËÃâ¿ë..
Àå°ü ÃâÇ÷¼º ´ëÀå±Õ ¿ë ..
Àå¿° Vibrio °ËÃâ¿ë Pri..
ÄÝ·¹¶ó µ¶¼Ò °ËÃâ¿ë Pri..
Ư¼ö ¼¼±Õ °ËÃâ¿ë Posit..
Ȳ»öÆ÷µµ»ó±¸±Õ °ËÃâ¿ë ..
Bacterial 16S rDNA PCR..
Bacterial 16S rDNA PCR..
Botulinus±Õ °ËÃâ¿ë Pri..
Fungal rDNA (ITS1) PCR..
O-157(º£·Î µ¶¼Ò À¯ÀüÀÚ..
Salmonella±Õ °ËÃâ¿ë Pr..
Shigella ¹× Àå°üħÀÔ¼º..
TaKaRa źÀú±Õ PCR Dete..
TaKaRa PCR Mycoplasma ..
Welchii±Õ °ËÃâ¿ë Prime..
Premix Taq Hot Start Version
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Hot Start PCR¿ëÀÇ DNA polymerase, ¹ÝÀÀ buffer, dNTP mixture¸¦ 2¹èÀÇ ³óµµ·Î È¥ÇÕÇÑ °ÍÀÌ´Ù. TaKaRa Ex Taq HS, TaKaRa Taq HS´Â anti-Taq antibody¿Í TaKaRa Ex Taq ¶Ç´Â TaKaRa TaqÀ» È¥ÇÕÇÑ Hot Start PCR ¿ë È¿¼Ò·Î °í¿ÂÀ¸·Î °¡¿ÇÒ ¶§±îÁö´Â anti-Taq antibody°¡ È¿¼Ò¿¡ °áÇÕÇØ polymerase È°¼ºÀ» ¾ïÁ¦ÇϹǷΠcycle ÀüÀÇ mis-priming°ú primer dimer¿¡¼ À¯·¡ÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù. Anti-Taq antibody´Â PCR ÃÖÃÊÀÇ DNA º¯¼º ´Ü°è¿¡¼ º¯¼ºÇϱ⠶§¹®¿¡ Á¾ÀüÀÇ PCR Á¶°ÇÀ¸·Î ¹ÝÀÀÇÒ ¼ö ÀÖ´Ù. Anti-Taq antibody Ç×ü¸¦ ½ÇÈ°½ÃÅ°±â À§ÇÑ Æ¯º°ÇÑ ´Ü°è´Â ÇÊ¿ä ¾ø´Ù.
Á¶¼º
TaKaRa Taq HS
1.25 units/25 ¥ìl
dNTP Mixture
2¡¿conc:°¢ 0.4 mM
PCR buffer
2¡¿conc:
- 20 mM Tris-HCl (pH8.9)
- 100 mM KCl
- 3 mM MgCl
2
PCR »ê¹°¿¡ ´ëÇØ
Premix Ex Taq Hot Start Version, Premix Taq Hot Start VersionÀ» ÀÌ¿ëÇؼ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3' ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector¿¡ Ŭ·Î´×ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, ¸»´Ü ÆòÈ°È ¹× Àλêȸ¦ ½Ç½ÃÇØ blunt endÀÇ vector·Î cloning ÇÒ ¼ö ÀÖ´Ù.
PCR °ËÁ¤
1. ¥ëDNAÀ» ÁÖÇüÀ¸·Î ÇÑ PCR ¹ÝÀÀ¿¡ ÀÖ¾î¼ ¾çÈ£ÇÑ ÁõÆøÀÌ È®ÀεǾú´Ù.
2. Human genomic DNA¸¦ ÁÖÇüÀ¸·Î ÇÑ single copy geneÀÇ PCR ¹ÝÀÀ(ÁõÆø»ê¹° 2.9 kb)¿¡ ´ëÇØ ¾çÈ£ÇÑ ÁõÆøÀ» È®ÀÎÇß´Ù.
PCR ¹ÝÀÀ¿¹ (total 50ul PCR)
Premix Ex Taq Hot Start Version or PremixTaq Hot Start Version*
25 ¥ìl
Template
<1 ¥ìg
Primer 1
0.2~1.0 ¥ìM£¨final conc.£©
Primer 2
0.2~1.0 ¥ìM£¨final conc.£©
¸ê±Õ Áõ·ù¼ö
up to 50 ¥ìl
* õõÈ÷ ¼¯¾î¼ ±ÕÀÏÇÏ°Ô È¥ÇÕÇؼ »ç¿ëÇÑ´Ù.
PCR Á¶°Ç
PCR ÃÖÃÊÀÇ DNA º¯¼º ´Ü°è¿¡¼ Anti-Taq antibody ½ÇÈ°(ã÷üÀ) µÇ¹Ç·Î Á¾ÀüÀÇ PCR Á¶°ÇÀ» »ç¿ëÇÒ ¼ö ÀÖ´Ù. Anti-Taq antibody¸¦ ½ÇÈ° ½ÃÅ°±â À§ÇÑ Æ¯º°ÇÑ ´Ü°è´Â ÇÊ¿ä ¾ø´Ù.
¿¹£© 1 kb DNA ÁõÆø½Ã
98¡É 10 sec, 55¡É 30 sec, 72¡É 1 min&nbs
Keyword : hot start PCR
Standard PCR: Ex, LA, rTaq
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
±ä ´ÜÆíÀÇ DNA / GC rich ¼¿ ÁõÆø
LA Taq
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
Recombinant Taq DNA Polymerase
Recombinant Taq
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)
¾÷±×·¹À̵å Taq HS Premix