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Home > ÀüÁ¦Ç°º¸±â > PCR > Standard PCR: Ex, LA, rTaq > Premix ½Ã¾à (Hot Start Version)
2¡¿ Premix PCR Solution

Premix ½Ã¾à (Hot Start Version)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R028A
Premix Taq¢â Hot Start Version (500 ¥ìl¡¿5 tube)
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
(50 ¥ìl PCR)
96,000¿ø 
120,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R028A_DS.v2110Da.pdf
Takara
RR030A
Premix Ex Taq¢â Hot Start Version (500 ¥ìl¡¿5 tube)
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
(50 ¥ìl PCR)
120,800¿ø 
151,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR030A_DS.v1902Da.pdf

Premix Taq Hot Start Version
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Hot Start PCR¿ëÀÇ DNA polymerase, ¹ÝÀÀ buffer, dNTP mixture¸¦ 2¹èÀÇ ³óµµ·Î È¥ÇÕÇÑ °ÍÀÌ´Ù. TaKaRa Ex Taq HS, TaKaRa Taq HS´Â anti-Taq antibody¿Í TaKaRa Ex Taq ¶Ç´Â TaKaRa TaqÀ» È¥ÇÕÇÑ Hot Start PCR ¿ë È¿¼Ò·Î °í¿ÂÀ¸·Î °¡¿­ÇÒ ¶§±îÁö´Â anti-Taq antibody°¡ È¿¼Ò¿¡ °áÇÕÇØ polymerase È°¼ºÀ» ¾ïÁ¦ÇϹǷΠcycle ÀüÀÇ mis-priming°ú primer dimer¿¡¼­ À¯·¡ÇÑ ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù. Anti-Taq antibody´Â PCR ÃÖÃÊÀÇ DNA º¯¼º ´Ü°è¿¡¼­ º¯¼ºÇϱ⠶§¹®¿¡ Á¾ÀüÀÇ PCR Á¶°ÇÀ¸·Î ¹ÝÀÀÇÒ ¼ö ÀÖ´Ù. Anti-Taq antibody Ç×ü¸¦ ½ÇÈ°½ÃÅ°±â À§ÇÑ Æ¯º°ÇÑ ´Ü°è´Â ÇÊ¿ä ¾ø´Ù.
Á¶¼º
TaKaRa Taq HS 1.25 units/25 ¥ìl
dNTP Mixture 2¡¿conc:°¢ 0.4 mM
PCR buffer 2¡¿conc:
- 20 mM Tris-HCl (pH8.9)
- 100 mM KCl
- 3 mM MgCl2
PCR »ê¹°¿¡ ´ëÇØ
Premix Ex Taq Hot Start Version, Premix Taq Hot Start VersionÀ» ÀÌ¿ëÇؼ­ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3' ¸»´Ü¿¡ A°¡ 1¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ ÀÌ PCR »ê¹°À» ±×´ë·Î T-vector¿¡ Ŭ·Î´×ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, ¸»´Ü ÆòÈ°È­ ¹× ÀλêÈ­¸¦ ½Ç½ÃÇØ blunt endÀÇ vector·Î cloning ÇÒ ¼ö ÀÖ´Ù.
PCR °ËÁ¤
1. ¥ëDNAÀ» ÁÖÇüÀ¸·Î ÇÑ PCR ¹ÝÀÀ¿¡ À־ ¾çÈ£ÇÑ ÁõÆøÀÌ È®ÀεǾú´Ù.
2. Human genomic DNA¸¦ ÁÖÇüÀ¸·Î ÇÑ single copy geneÀÇ PCR ¹ÝÀÀ(ÁõÆø»ê¹° 2.9 kb)¿¡ ´ëÇØ ¾çÈ£ÇÑ ÁõÆøÀ» È®ÀÎÇß´Ù.
PCR ¹ÝÀÀ¿¹ (total 50ul PCR)
Premix Ex Taq Hot Start Version or PremixTaq Hot Start Version* 25 ¥ìl
Template <1 ¥ìg
Primer 1 0.2~1.0 ¥ìM£¨final conc.£©
Primer 2 0.2~1.0 ¥ìM£¨final conc.£©
¸ê±Õ Áõ·ù¼ö up to 50 ¥ìl
* õõÈ÷ ¼¯¾î¼­ ±ÕÀÏÇÏ°Ô È¥ÇÕÇؼ­ »ç¿ëÇÑ´Ù.
PCR Á¶°Ç
PCR ÃÖÃÊÀÇ DNA º¯¼º ´Ü°è¿¡¼­ Anti-Taq antibody ½ÇÈ°(ã÷üÀ) µÇ¹Ç·Î Á¾ÀüÀÇ PCR Á¶°ÇÀ» »ç¿ëÇÒ ¼ö ÀÖ´Ù. Anti-Taq antibody¸¦ ½ÇÈ° ½ÃÅ°±â À§ÇÑ Æ¯º°ÇÑ ´Ü°è´Â ÇÊ¿ä ¾ø´Ù.
¿¹£© 1 kb DNA ÁõÆø½Ã
98¡É 10 sec, 55¡É 30 sec, 72¡É 1 min&nbs

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